一、产品名称

尊龙凯时 Anti-DNA-RNA杂交抗体 (S96) (RGK60001)

二、产品描述

DNA-RNA杂交体是一种在真核细胞中普遍存在的自然现象，特别是在高转录活性位点，其表达水平较高。这些杂交体属于非经典的核酸结构，发挥着重要的转录调控功能。研究表明，它们的存在可能会增加特定基因座发生染色体断裂的风险。DNA:RNA杂交的形成会导致双链A/B中间构象的出现，为替代的DNA链上的单链核酸结合蛋白提供了结合靶点。同时，这些杂交体对DNA甲基转移酶的活性具有一定的抗性，并与多种神经系统疾病的发生有相关性。例如，DNA:RNA解旋酶Senataxin（SETX）的突变与显性青少年肌萎缩侧索硬化症4型和隐性眼动失协调2型密切相关。

三、产品详情

尊龙凯时 Anti-DNA-RNA杂交抗体 (S96) (RGK60001) 货号：RGK60001 表达系统：哺乳动物细胞 种属反应性：广谱 宿主：小鼠 同种型：IgG2a, kappa 克隆类型：单克隆 靶标：DNA-RNA杂交 浓度：0.58 mg/ml 内毒素水平：请联系实验室获取该信息 纯度：95%（依SDS-PAGE测定） 纯化方式：从细胞培养上清液中通过Protein A/G纯化 克隆号：S96 应用：ChIP, ChIP-seq, DB, EMSA, FISH, ICC, IF, IP, SPR 状态：液体 保存溶液：0.01 M PBS, pH 7.4 稳定性和储存：建议使用手动解冻冷冻器，避免反复冻融 短期存储：4°C（1-2周） 中期存储：-20°C（12个月） 长期存储：-80°C

四、参考文献

1. Recognition of RNA by the S96 antibody creates pervasive artifacts when imaging RNA:DNA hybrids PMID:33830170 2. Immunoprecipitation of DNA:RNA Hybrids Using the S96 Antibody PMID:32681514 3. High-resolution, strand-specific R-loop mapping via S96-based DNA-RNA immunoprecipitation and high-throughput sequencing PMID:31053798 4. RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage PMID:29742442 5. The monoclonal S96 antibody exhibits highly variable binding affinities towards different R-loop sequences PMID:28594954 6. Structural basis of R-loop recognition by the S96 monoclonal antibody PMID:35347133 7. Best practices for the visualization, mapping, and manipulation of R-loops PMID:33411340 8. Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging PMID:34232287 9. The Affinity of the S96 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast PMID:29289567 10. The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S96 PMID:23784994 11. Modifications and interactions at the R-loop PMID:32961406 12. BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2 PMID:24896180 13. R-Loop Analysis by Dot-Blot PMID:33554969 14. Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead PMID:34324345 15. New insight into the biology of R-loops PMID:32516653 16. Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR PMID:33371452 17. Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation PMID:32105733 18. Complexities due to single-stranded RNA during antibody detection of genomic RNA:DNA hybrids PMID:25890199 19. Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening PMID:34495394

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